Zebrafish facility

Zebrafish is one of leading models for studying the embryonic development, for modeling human diseases and for toxicological tests.

Zebra

This model presents many advantages:

  • Very rapid embryonic development (all major organs are formed within 3 days)
  • Transparency of the embryos (allowing visual observations of internal structures and non-invasive imaging)
  • Short generation time (sexually mature after 3-4 months)
  • High fecundity (a single female can lay up to 150 eggs per clutch and per week allowing powerful statistics)
  • High genetic homology to humans (about 75% of the human genome has functional homologies with zebrafish)

These characteristics make it an appropriate model for the study of developmental processes, drug discovery and screening, neurosciences and cardiovascular research, wound healing, microbe-host interactions, behavior genetics, toxicology…

Logo_Logo-autres_GIGAThe GIGA zebrafish platform offers a broad range of services from routine to tailor-made such as:

  • CRIPSR mutants and morphants generation (in vivo functional characterization of gene function and validation of candidate genomic sequences)
  • Toxicology and efficacy tests: teratogenicity, acute toxicity test (LC50)…
  • Housing and zebrafish training
  • Sperm cryopreservation, in vitro fertilization and biobanking
  • Studies with the zebrabox, an automated video tracking system that provides high-throughput behavioral analysis data
  • Tailor-made zebrafish projects/consultancy services

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

  • 600 tanks split up into 3 separate multi-rack systems and 3 stand-alone systems
  • 15000 zebrafish
  • Six microinjection platforms for transgenesis and mutants generation
  • Fluorescence microscopes and stereomicroscopes with camera and image analysis software
  • Motorized advance Vibroslice Campden MA752
  • Automated whole mount in situ hybridization station
  • Zebrabox: High-throughput solution for behavioral analysis data

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Mouse facility

Picture_Mouse facility_Ulg_Animal_CHU (2)In applied research, mouse models remain a mandatory step to evaluate the efficiency and toxicity of potential treatments.

The short live cycle and a high prolific make mice a very interesting model. The mouse genome is well-known and shows a high homology with human.

Transgenic mice constitute a potential tool for deciphering the role of the targeted gene.

 

The Specific Pathogen-Free (SPF) mouse facility and transgenic platforms offer a broad range of services:

  • Housing (conventional or specific pathogen-free facilities)
  • Experimental behavior recording
  • Animal monitoring
  • Transgenesis
  • Line cryopreservation
  • Mice rederivation by embryo transfer
  • Mouse Embryo Assays (MEA)
  • Murine Local Lymph Node Assay (LLNA)
  • In vivo imaging (for more information, please click here)
  • Microscopic surgery
  • Toxicology
  • On-demand studies

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Our equipment include:

  • Echocardiography (GE Healthcare)
  • X-RAD 225CX Small animal guided-irradiator (for more information, click here)
  • Computer-guided stereotaxic frame designed for rodents
  • Comprehensive Lab Animal Monitoring System (Oxymax-CLAMS Metabolic system) provides an adaptable test environment with snap-in modules to support a broad array of physiological and behavioral monitoring capabilities such as activity (X, Y & Z axis monitoring), feeding (mass monitoring), drinking (volume monitoring, running wheel (rotation monitoring), sleep detection (scores events and duration, food access control (mass & event monitoring), calorimetric assessment (by Oxymax), temperature & heart rate (by telemetry), environmental Control (temperature & lighting) & monitoring
  • > 60 different analyzes can be performed in animal serum, plasma and urine samples thanks to a dedicated automated analyzer equipped for colorimetry, turbidimetry, latex agglutination, homogeneous enzyme immunoassay (EIA) and indirect ion selective electrode (ISE)
  • Fully-equipped preclinical imaging platform (for more information, click here)
  • Fully-equipped lab for surgery and dissection
  • Fully-equipped lab for embryo manipulations, storage and cell culture

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.