Mass Spectrometry

Using state-of-the-art equipment, researchers of ULg provide innovative solutions in the fields of:

  • Analytical chemistry (volatile and non-volatile small organic compound analysis…)
  • Medical chemistry (toxicology analysis in biological fluids, identification and quantification of new biomarkers, biomarker signature validation…)
  • Biological and pharmaceutical chemistry (biomolecule full characterization, pharmaceutical interaction with protein or DNA target, quantification of metabolites of a new drug…)
  • Biophysics and structural chemistry (structure of supra-molecular assemblies…)

Moreover, our lab investigate and develop new mass spectrometric methodologies such as new ion excitation methods using photons, electrons or hydrogen radicals.

 

We can develop new analytical methods for the determination and reliable quantification of a wide range of small organic molecules (ranging from 100 to 600 Da), whether volatile, semi-volatile, non-volatile or thermos labile, as long as the compound is available as a standard.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

We are accredited for dioxin and PCB analysis in several matrices like blood, food, milk, etc.

We participate to food analysis campaigns since the Belgian dioxin crisis in 1999 and contribute to the development of efficient sample preparation techniques for faster analyses.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Laser capture microdissection

Picture5Tissue microdissection or laser capture microdissection (LCM) is an automated sample preparation technique enabling the isolation of specific cells from heterogeneous materials under microscopic visualization. LCM is contact-free, thanks to the specimen collection by gravity, which helps to minimize contamination. This precise technique allows the selective analysis of specific regions, down to single cells and the extraction of their proteins and nucleic acids.

LCM allows to obtain answers in a single cell that may otherwise have been missed in a whole tissue scrape that contains a heterogeneous mixture of healthy, stromal and cancer cells. This technique of isolating a pure sample from a heterogeneous mixture allows more efficient and accurate results with downstream microgenomics applications such as next-generation sequencing, Sanger sequencing, PCR and proteomics.

Our specific equipment includes a LDM 7000 LEICA.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Proteomics

Our Proteomic GIGA platform is at the forefront of technological innovation and assay development. The facility is hosted by the Mass Spectrometry Laboratory and works in collaboration with the Center for Analytical Research and Technology.

It offers expert services in proteins characterization (sequencing, post-translational modifications, heterogeneity, etc) and targeted quantification (biologics, biomarkers).

 

We have a high level of expertise in:

  • Protein identification and subsequent analysis of peptides by:
    • LC-MS/MS
    • MALDI-TOF/TOF
  • Protein sequencing and characterization by:
    • MELD digestion followed by LC-MS/MS
    • Top-down sequencing
  • Molecular mass determination of small molecules or purified homogeneous proteins by:
    • ESI-MS-TOF
    • MALDI-MS
    • FT-ICR
  • Quantitative proteomics:
    • Label-free differential proteomics (relative quantification)
    • Absolute quantification
  • Quality control: purity, quality and protein component(s) identification
  • Miscellaneous:
    • Molecular imaging (more info here)
    • Protein purification (more info here)
    • Gel electrophoresis

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

TOF instruments:

  • ESI-Synapt G2 HDMS (Waters)
  • MALDI UltrafleXtreme TOF/TOF (Bruker)
  • ESI-Synapt G1 HDMS (Waters)
  • ESI-Q-TOF Ultima Global (Waters)
  • ESI-Micro TOF (Bruker)

FT instruments:

  • Q Exactive Plus and Q exactive (Thermo scientific)
  • ESI/MALDI-FT-ICR MS SolariX 9.4T (Bruker)

Ion Trap Instrument (ESI-Ion trap AmaZon Speed ETD (Bruker))

Triple quadrupole instruments:

  • ESI-Xevo TQ-S (Waters)
  • ESI-Quattro Ultima Pt (Waters)

HPLC/UPLC instruments:

  • Two 2D NanoAcquity UPLC (Waters)
  • 2D-nano-HPLC Ultimate 3000 (Dionex)
  • Two Acquity UPLC I-Class (UV detector available, Waters)
  • MicroUPLC M-class (Waters)
  • TriVersa NanoMate (Advion)

Sample preparation:

  • Janus liquid handling workstation (Perkin Elmer)
  • Ettan Dalt six, IPGphore, Spot picker and Typhoon scanner TM9400 (GE Healthcare)
  • Probot fraction collector and MALDI spotter (Dionex)
  • Gelfree 8100 (Expedeon)
  • ImagePrep (Bruker)
  • SunCollect (SunChrom)

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Since 2012, we also have FAMHP agreement for qualitative analyses of proteins.

Genomics/Transcriptomics

Our Genetics GIGA platform is at the forefront of technological innovation and assay development. It offers expert services in high-throughput genomic assays, including array- and NGS-based NSP genotyping, variant detection, transcriptome and epigenome analyses.

In addition, the Genetic Department of the CHU has developed a large expertise in the diagnosis of genetic diseases, including genetic predisposition to cancer, as well as the diagnosis of acquired genetic and epigenetic features in cancers in pediatric and adult patients, and in the analysis of acquired genetic anomalies in diverse malignant tumors (leukemia’s, lymphomas and various solid tumors). The genetic department is one of the 8 Centers for Human Genetics in Belgium and we are BELAC accredited for diagnostic tests for genetic diseases and cancer (ISO 15189).

We are ready to help you in various projects such as patient characterization, metabolic profiling during clinical studies, exploration of SNPs or high throughput DNA sequencing in search for treatment targets and/or markers predictive of response or resistance.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Our services and capabilities include:

  • DNA extraction/purification from a wide range of sources
  • QC of DNA/RNA
  • Gene expression profiling
  • Sanger cycle sequencing based on the Sanger method
  • Next generation sequencing based on Illumina technology
  • Genomic sequencing, including variant detection (SNP, insertions, deletions and genomic rearrangements)
  • RNA sequencing
  • Targeted sequencing
  • Sequencing of enriched DNA fractions
  • Bisulfite sequencing
  • Genotyping ranging from low throughput to high throughput
  • Automated DNA and RNA extraction

The following equipment is available:

  • Next generation sequencing: complete ILLUMINA platform HiSEQ2000, MiSEQ, NextSEQ500 (acquired at the end of 2014)
  • Sanger sequencing: ABI 3730 automatic capillary sequencer
  • Denaturing High Performance Liquid Chromatography (dHPLC)
  • Mass spectrometry (MS/MS)
  • Multiplex Ligation-dependent Probe Amplification (MLPA)
  • Comparative Genomic Hybridization on microarray (CGH array) (Agilent 2100 Bioanalyzer)
  • Array genotyping: ILLUMINA Iscan with Autoloader
  • qPCR: ABI 7900HT with an Autoloader
  • DNA standard sequencer

Metabolomics

Our lab are equipped with state-of-the-art technologies to detect, separate, identify and quantify molecules covering a large chemical space. Non-target and target analyses are performed using Nuclear Magnetic Resonance (NMR) and chromatography coupled to mass spectrometry. Researchers at ULg and CHU have extensive experience working with different matrices such as blood, plasma, milk, urine, saliva, organs/tissues, cell lysates, oils, growth media, plant extracts…

Metabolomics analysis can accurately determine the slightest changes of a molecular profile related to disease onset and progression, therapeutic intervention, genetic modification or environmental variation.

We provide following services:

  • Metabolomics analysis of biofluids (sera, plasma, urine…), tissues, cells, bioproducts and plant extracts
  • Metabolic profil studies of drug metabolites
  • Structural determination of unknown metabolites
  • Biomarker discovery

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Our specific equipment and techniques include:

  • Nuclear magnetic resonance (NMR) Bruker Avance 500 MHz with a TCI Cryoprobe
  • Gilson sample preparation
  • Liquid Chromatography-Solid Phase Extraction (LC-SPE) system
  • Comprehensive two-dimensional GC (GCxGC) coupled to time-of-flight MS (TOFMS):
    • Cryogenic modulators on low resolution LECO Pegasus 4D and DANI MasterTOF
    • Cryogenic modulator on high resolution JEOL ACCUTOF GC2G
    • Cryo-free modulator on high resolution JEOL ACCUTOF GCv4G

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.