Protein characterization

Picture_Logo_CIPWe offer biochemical and biophysical analyses to assess the purity, folding and stability of the purified sample. This includes:

  • Biochemical characterization (enzymology, kinetics, sequencing, protein-protein interactions, proteomics, etc)
  • Biophysical charaterization (microcalorimetry, dynamic/static light scattering, protein stability, folding & aggregation, X-ray crystallography, etc)
  • Our Robotein Platform encompasses an Octet system that allows high-throughput analyses of protein interactions in 96- and 384-well microplates. It enables real-time and label-free determination of affinity, kinetics and concentration. This makes it highly suitable for the characterization of drug candidates. Rapid screening and epitope binding assays are enabled by the multiplexed biosensor formats

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.


Protein production & purification

Picture_Logo_CIPThe protein factory platform provides many services as like:

  • Genetic engineering (site-directed mutagenesis, random mutagenesis, protein design) and cloning in bacterial strains such as Coli, Bacillus subtilis, Bacillus amyloquefaciens, Streptomyces lividans or in yeast such as Pichia pastoris
  • Analytical scale or pilot scale recombinant protein production in high cell density conditions
  • Cell harvesting or supernatant cleaning using bucket or continuous centrifugation or hollow fiber filtration
  • Cell disintegration to recover proteins produced in the intracellular compartment
  • Protein purification at analytical and pilot scales