Rotary Cell Culture System

The rotary cell culture system is a device designed to grow 3D cells clusters (tissue, cancer tumors and virus cultures) in microgravity (outside the body). The advantage here is to obtain larger and 3D cell cultures with structural and chemical characteristics similar to normal tissue, in comparison with lab-grown cell cultures. It allows to test […]

2D-LEC cultures

In these assays, (Lymphatic) Endothelial Cells ((L)ECs) are seeded as monolayers on culture plates or onto the surface of matrix-coated plates. Such 2D cultures can be used to evaluate the effects of putative lymphangiogenic stimulators or inhibitors on specific LEC properties. While none of the 2D cultures can undergo all steps of lymphatic vessel formation, […]

3D-LEC Cultures

3D cultures bridge the gap between in vitro and in vivo assays and are available to follow the sprouting process and Lymphatic Endothelial Cells (LEC) morphogenesis. The lymphatic-ring assay appears as a potent tool for the study of pathological lymphangiogenesis, defined as the abnormal formation of new lymphatic vessels from preexisting ones. Contact [] us to discuss […]

High-grade glioma-initiating cells derived from normal astrocytes

The transformed astrocytes are obtained from normal mouse astrocytes undergoing gamma irradiation after TGF-alpha treatment. In this in vitro model, transformation causes major losses of astrocyte-specific proteins and functions and the acquisition of metabolic adaptations that favor intermediate metabolites production for increased macromolecule biosynthesis. The cells display all canonical features of cancerous transformation, immortalization, uncontrolled growth, cytogenetic […]

Clonogenic assay

Clonogenic assay or colony formation assay is an in vitro cell survival assay based on the ability of a single cell to grow into a colony. The colony is defined to consist of at least 50 cells. This assay essentially tests every cell in the population for its ability to undergo “unlimited” division. Clonogenic assay […]

Chondrocytic dedifferentiation model

Although dedifferentiation is a phenomenon described for decades, molecular events underlying the process are not yet fully elucidated. As a consequence, dedifferentiation still constitutes a major brake in vitro for chondrocytes expansion, thereby preventing cartilage regeneration using expanded autologous chondrocytes. To provide better understanding of chondrocytes biology, the researchers at GIGA applied human osteoarthritis hip […]

Mesenchymal differentiation model

The researchers from GIGA have a large expertise in the isolation, expansion and differentiation of mesenchymal synovial fibroblasts (SVF) isolated from synovial tissue. SVF monolayer cultures represent an interesting model to study adipogenic, chondrogenic and osteogenic properties. The isolated SVF: Are plastic adherent cells with an important proliferation potential Express mesenchymal stem cell immunophenotype (CD105, […]

Osteoblast-like cells cultures in monolayer

In this model, osteoblast-like cells lines are cultured in monolayer. It allows a better understanding of signaling pathways in various experimental conditions or after exposure to various agents. Contact [] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Tumor model on CAM

In this model, glioblastoma cells are implanted on chicken chorioallantoic membrane (CAM) at day 11 after fertilization. This implantation results in tumor growth showing an histology similar to glioblastoma multiform in patients, with diffuse pleiomorphic infiltrate of fibrillar and stellate cells, neoangiogenesis, edema, and areas of necrosis. The growth of tumors on CAM provides a […]

Organotypic culture models

Organotypic culture involves the combination of cells in a specific ratio to create a component of an organ. Researchers at GIGA have expertise in development of organotypic cultures of normal and tumor epithelial cells, involving the growing cells in a 3D environment. They developed organotypic culture systems that permit epithelial cells to proliferate and differentiate […]