Key regulators of bone formation are highly conserved between mammals and teleosts. The corresponding orthologs share significant sequence similarities and an overlap in expression patterns when compared to mammals. Cranial cartilage is the first skeletal structure to be detected as early as 3 days post-fertilization, while first calcified intramembranous bone structures start to form at about […]
Lung colony assay is a technique to assay the clonogenicity of the cells of a solid tumor irradiated in situ by injecting them into recipient animals and counting the number of lung colonies produced. Contact [firstname.lastname@example.org] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.
Researchers at GIGA have developed a murine graft-versus-myeloma (GVM) model by combining an immunocompetent myeloma model and a chronic graft-versus-host (GVH) disease model. This model is currently used for further studies aiming at dissociating GVM and GVH effect. Contact [email@example.com] us to discuss how these capabilities can forward your projects! We will help you develop tailored […]
The main features of temporal lobe epilepsy (TLE) are: Epileptic foci in the limbic system An “initial precipitating injury” The so-called “latent period” The presence of hippocampal sclerosis leading to reorganization of neuronal networks Many of these characteristics can be reproduced in rodents by systemic injection of pilocarpine. In this animal model, status epilepticus is […]
“Tumor graft models” (also known as Patient-Derived Xenografts or PDXs) are based on the transfer of primary tumors directly from the patient into an immunodeficient mouse. To accomplish this, patient tumors must be obtained fresh from surgery. Tumors can be engrafted heterotopically or orthotopically. PDX models may be superior to traditional cell line – xenograft […]
In this model, human cancer cells are transplanted either heterotopically (into the subcutaneous flank) or orthotopically (direct implantation to the mouse organ from which the tumor is originated) into immunocompromised mice. The response to appropriate therapeutic regimes (such as anti-angiogenic drugs) on tumor size (diameter, area or volume) and animal survival (determined at regular intervals) can be pursued. The in […]
Previous studies have demonstrated the importance of Dicer during early cochlear development. To extend this investigation to the establishment and differentiation of the prosensory domain, researchers at GIGA crossed Dicerflox/flox mice with FoxG1Cre/+ mice to generate embryos (i.e., FoxG1:Dicer-cKO) with an early (E8.5) depletion of Dicer in the otocyst. Contact [firstname.lastname@example.org] us to discuss how these […]