Tumor growth

Angiogenesis

Zebrafish Model

Key regulators of bone formation are highly conserved between mammals and teleosts. The corresponding orthologs share significant sequence similarities and an overlap in expression patterns when compared to mammals.

Cranial cartilage is the first skeletal structure to be detected as early as 3 days post-fertilization, while first calcified intramembranous bone structures start to form at about the same time.

Due to its rapid generation time, large offspring numbers, external development, transparency and the availability of genetic maps, the zebrafish is a very attractive model system to study the function of genes involved in bone formation.

Transgenic zebrafish lines represent unique tools to follow osteoblasts in vivo and to analyze their function in wild type or mutant backgrounds.

This animal model can be used to evaluate drug or treatment effects on cartilage and bone formation by measuring:

  • The intensity and progression of bone formation
  • Their level of ossification
  • The level of calcification
  • Morphometric analysis

Using this model, researchers from GIGA have evaluated the physiological consequences of altered gravity on bone formation and more generally on whole genome gene expression.

For more info on our zebrafish facility, click here.

Lung colony assay

Lung colony assay is a technique to assay the clonogenicity of the cells of a solid tumor irradiated in situ by injecting them into recipient animals and counting the number of lung colonies produced.

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GVM Mouse model

Researchers at GIGA have developed a murine graft-versus-myeloma (GVM) model by combining an immunocompetent myeloma model and a chronic graft-versus-host (GVH) disease model. This model is currently used for further studies aiming at dissociating GVM and GVH effect.

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pilocarpine mouse model

The main features of temporal lobe epilepsy (TLE) are:

  • Epileptic foci in the limbic system
  • An “initial precipitating injury”
  • The so-called “latent period”
  • The presence of hippocampal sclerosis leading to reorganization of neuronal networks

Many of these characteristics can be reproduced in rodents by systemic injection of pilocarpine.

In this animal model, status epilepticus is followed by a latent period and later by the appearance of spontaneous recurrent seizures.

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Transgenic mice

PDX mouse model

“Tumor graft models” (also known as Patient-Derived Xenografts or PDXs) are based on the transfer of primary tumors directly from the patient into an immunodeficient mouse.

To accomplish this, patient tumors must be obtained fresh from surgery. Tumors can be engrafted heterotopically or orthotopically.

PDX models may be superior to traditional cell line – xenograft models of cancer because they maintain more similarities to the parental tumors. Detailed examination of PDX mice indicate that histology and gene expression profiles are retained, along with SNPs and copy number variants.

PDX models are maintained by passaging cells directly from mouse to mouse once the tumor burden becomes too high.

PDX models offer a powerful tool for studying tumor biology and for evaluating anticancer drugs.

The in vivo imaging system (Xenogen®) can be used to better follow tumor progression. For more information, click here.

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Tumor xenograft models

In this model, human cancer cells are transplanted either heterotopically (into the subcutaneous flank) or orthotopically (direct implantation to the mouse organ from which the tumor is originated) into immunocompromised mice.

The response to appropriate therapeutic regimes (such as anti-angiogenic drugs) on tumor size (diameter, area or volume) and animal survival (determined at regular intervals) can be pursued.

The in vivo imaging system (Xenogen®) can be used to better follow tumor progression. For more information, click here.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Model of Dicer depletion in the otocyst

Previous studies have demonstrated the importance of Dicer during early cochlear development.

To extend this investigation to the establishment and differentiation of the prosensory domain, researchers at GIGA crossed Dicerflox/flox mice with FoxG1Cre/+ mice to generate embryos (i.e., FoxG1:Dicer-cKO) with an early (E8.5) depletion of Dicer in the otocyst.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.