Effects of microgravity simulation on zebrafish transcriptomes and bone physiology

Effects of microgravity simulation on zebrafish transcriptomes and bone physiology

Physiological modifications in near weightlessness, as experienced by astronauts during space flight, have been the subject of numerous studies. Various animal models have been used on space missions or in microgravity simulation on ground to understand the effects of gravity on living animals. Here, we used the zebrafish larvae as a model to study the effect of microgravity simulation on bone formation and whole genome gene expression. To simulate microgravity (sim-μg), we used two-dimensional (2D) clinorotation starting at 5 days post fertilization to assess skeletal formation after 5 days of treatment. To assess early, regulatory effects on gene expression, a single day clinorotation was performed. Clinorotation for 5 days caused a significant decrease of bone formation, as shown by staining for cartilage and bone structures. This effect was not due to stress, as assessed by measuring cortisol levels in treated larvae. Gene expression results indicate that 1-day simulated microgravity affected musculoskeletal, cardiovascular, and nuclear receptor systems. With free-swimming model organisms such as zebrafish larvae, the 2D clinorotation setup appears to be a very appropriate approach to sim-μg. We provide evidence for alterations in bone formation and other important biological functions; in addition several affected genes and pathways involved in bone, muscle or cardiovascular development are identified.

References :

Effects of microgravity simulation on zebrafish transcriptomes and bone physiology—exposure starting at 5 days post fertilization

Jessica Aceto, Rasoul Nourizadeh-Lillabadi, Silvia Bradamante, Jeanette A Maier, Peter Alestrom, Jack JWA van Loon & Marc Muller

Article number: 16010 (2016) doi:10.1038/npjmgrav.2016.10

Aceto J, Nourizadeh-Lillabadi R, Maree R, Dardenne N, Jeanray N, Wehenkel L, Alestrom P, van Loon JJ, Muller M (2015). Zebrafish bone and general physiology are differently affected by hormones or changes in gravity. PLoS One 10,e0126928.

Related articles

A new gene responsible for juvenile myoclonic epilepsy?

In collaboration with a team at the University of California at Los Angeles (UCLA), researchers from GIGA-Neurosciences have discovered a new gene responsible for a seizure syndrome called juvenile myoclonic epilepsy (JME). This discovery was made as part of an international consortium that studies genetic abnormalities responsible for epileptic diseases. It is being published this week in

Read more
A new gene responsible for juvenile myoclonic epilepsy?

New technologies available at the GIGA Imaging platform

The LIGHTSHEET MICROSCOPY can deliver optical sections, 3D reconstructions and timelapse movies of whole sample volumes at subcellular resolutions. The fast scan speeds and low phototoxicity of the lightsheet allow to record the development of fluorescent transgenic animals over long time periods, such as zebrafish embryos. Alternatively 3D reconstructions of fixed whole organs or whole embryos,

Read more
New technologies available at the GIGA Imaging platform

A team from the University of Liège has discovered a new crosstalk between the migrating inhibitory interneurons and the stem cells that generate the excitatory neurons

The researchers discovered that this cellular dialogue controls the growth of the cerebral cortex and that its impairment leads a cortical malformation previously associated with autism in mice . Their results are published in the prestigious scientific journal Cell. The cerebral cortex contains excitatory and inhibitory interneurons. The former are produced locally and move by

Read more
A team from the University of Liège has discovered a new crosstalk between the migrating inhibitory interneurons and the stem cells that generate the excitatory neurons