wound scratch assayIn these assays, (Lymphatic) Endothelial Cells ((L)ECs) are seeded as monolayers on culture plates or onto the surface of matrix-coated plates.

Such 2D cultures can be used to evaluate the effects of putative lymphangiogenic stimulators or inhibitors on specific LEC properties. While none of the 2D cultures can undergo all steps of lymphatic vessel formation, these systems contribute to analyze cell activities (e.g. gene expression profiling), cell proliferation, apoptosis, adhesion, migration (wound scratch assay, boyden chamber assay) and morphogenesis (tubulogenesis).

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