The choroid-allantoic chicken membrane (CAM) assay constitutes an easy and affordable system for preclinical characterization that may precede the mandatory process of animal testing.

The tested substance is prepared either in slow-release polymer, or absorbed by gelatin sponges, or air-dried onto plastic discs; which are then implanted onto the CAM through a window cut carefully in the eggshell.

The lack of a mature, immune system in 7–8-day old chick embryos allows the study of tumor-induced angiogenesis.

The angiogenic effects can be measured by counting the number of blood vessels in a given area.

This models is also useful to study the tumor growth.

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