Organotypic culture involves the combination of cells in a specific ratio to create a component of an organ. Researchers at GIGA have expertise in development of organotypic cultures of normal and tumor epithelial cells, involving the growing cells in a 3D environment.
They developed organotypic culture systems that permit epithelial cells to proliferate and differentiate at an air – liquid interface on a dermal equivalent support. Normal keratinocytes stratify and fully differentiate in a manner similar to the normal squamous epithelial tissues.
This culture system is more similar biochemically and physiologically to in vivo tissue. Human epidermis reconstructed in vitro may be used as the best alternative for the in vitro testing of the toxicology and efficiency of products for topical use, as well as in the treatment of skin burns and chronic skin ulcers.
The developed models include: