In this model, glioblastoma cells are implanted on chicken chorioallantoic membrane (CAM) at day 11 after fertilization. This implantation results in tumor growth showing an histology similar to glioblastoma multiform in patients, with diffuse pleiomorphic infiltrate of fibrillar and stellate cells, neoangiogenesis, edema, and areas of necrosis.

The growth of tumors on CAM provides a more rapid, low cost, and ethically sustainable alternative.

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