Mechanical stretch on valvular interstitial cells
The effect of the mechanical stretch on valvular interstitial cells can be analyzed using the Flexercell® FX4000™ Tension System, a computer-regulated bioreactor.
This system utilizes regulated vacuum pressure to apply cyclic or static strain to cells, cultured on flexible-bottomed culture plates. It helps to study the effects of mechanical forces of various intensities and frequencies on the phenotype of adherent cultured cells. Moreover, different types of forces (tension, shear stress, compression) can be applied in 2D or 3D models.
Coagulation & thrombin functional tests
Those analyses are performed using following tests and equipment:
- BCS and ACL Top coagulometers to evaluate clotting factors, anticoagulants, therapeutic monitoring and activation markers
- Thromboelastrometry TEM® to measure the interaction of coagulation factors, inhibitors and cellular compounds during the phases of clotting and subsequent lysis over time
- Calibrated Automated Thrombinography (CAT) to measure thrombin generation by adding a fluorogenic substrate
- Von Willebrand factor tests
Platelets Functional tests
These experiments can be performed using following equipment:
- Cell-Dyn 3700 hemocytometer with human and veterinary software
- Chronolog 700 lumi-agreggometer to measure platelet function with simultaneously measuring ATP release. It also has the capability to perform the Ristocetin Cofactor assay
- Multiplate determination of platelet aggregation in blood through electrical impedance method
- PFA-100 (Siemens) to determine platelet function in flow conditions
- Impact-R cone and plate viscometer (Diamed) to determine adhesion and aggregation in flow conditions
- Flow cytometry
- Confocal microscopy
Oxygen consumption rate
The oxygen consumption rate of different cells types can be measured and the modulating effect of molecules (natural or synthetic) on mitochondrial respiratory parameters, mitochondrial function/dysfunction can be evaluated using an Ouroboros Oxygraph-2k, a modular system for high-resolution respirometry.