High-grade glioma-initiating cells derived from normal astrocytes

The transformed astrocytes are obtained from normal mouse astrocytes undergoing gamma irradiation after TGF-alpha treatment.

In this in vitro model, transformation causes major losses of astrocyte-specific proteins and functions and the acquisition of metabolic adaptations that favor intermediate metabolites production for increased macromolecule biosynthesis.

The cells display all canonical features of cancerous transformation, immortalization, uncontrolled growth, cytogenetic alterations, and give rise to high-grade gliomas when injected into nude mice. In culture, they constitute a homogenous population of transformed astrocytes particularly suited for quantitative biochemical comparison with their normal counterparts, represented by primary cultures of astrocytes. It could similarly be applied to the evaluation of the effects of treatments aimed at correcting the consequences of cell transformation.

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Tumor model on CAM

In this model, glioblastoma cells are implanted on chicken chorioallantoic membrane (CAM) at day 11 after fertilization. This implantation results in tumor growth showing an histology similar to glioblastoma multiform in patients, with diffuse pleiomorphic infiltrate of fibrillar and stellate cells, neoangiogenesis, edema, and areas of necrosis.

The growth of tumors on CAM provides a more rapid, low cost, and ethically sustainable alternative.

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Glioblastoma cell lines

We have multiple cell lines at our disposal, highly suited for glioblastoma research such as:

  • Primary glioblastoma cultures established acutely resected human glioblastoma
  • Human glioblastoma cell lines (U87MG ATCC HTB-14, U373, U251-MG, LN18)

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Oxygen consumption rate

The oxygen consumption rate of different cells types can be measured and the modulating effect of molecules (natural or synthetic) on mitochondrial respiratory parameters, mitochondrial function/dysfunction can be evaluated using an Ouroboros Oxygraph-2k, a modular system for high-resolution respirometry.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.