pilocarpine mouse model

The main features of temporal lobe epilepsy (TLE) are:

  • Epileptic foci in the limbic system
  • An “initial precipitating injury”
  • The so-called “latent period”
  • The presence of hippocampal sclerosis leading to reorganization of neuronal networks

Many of these characteristics can be reproduced in rodents by systemic injection of pilocarpine.

In this animal model, status epilepticus is followed by a latent period and later by the appearance of spontaneous recurrent seizures.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Stability testing

Stability testing is performed after storage in defined conditions according to standard and accelerated ICH conditions. We provide you with different range of storage conditions. All storage rooms are fully-controlled with 24/7 monitoring and alert systems.

Our services include:

  • Support in designing studies for real-time
  • Development and validation of “stability indicating methods”
  • Examination of stability-relevant parameters (temperature – humidity)
  • Interim reports for every testing period
  • Comprehensive final report

Looking for clinical advice or support, or preparing for a clinical study? We will help you set up a cost-effective and time-efficient study! Get in touch with our experts [info@b2h.be].

Potassium channel modulators

The new compounds based on disubstituted anilines, by opening potassium channels, can be used for the treatment of vasospastic disorders (i.e. subarachnoid hemorrhage and migraine), of various diseases of the central nervous system (i.e. epilepsy, ischemia and neurodegenerative diseases) as well as pain treatment.

Interested in these projects? Drop us an email [info@b2h.be]. We are looking forward collaborating with you!

Drug administration & Patient monitoring

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  • Centralized clinical unit with 30 equipped hospital beds
  • Experience in running different study types and trial design
  • Different types of drugs like NCE and NBE
  • Database of more than 2.700 healthy and reliable volunteers
  • Privileged access to medical and technical expertise of CHU and ULg (such as Cyclotron, GIGA, CIP, Sleep Center, Pain clinic…)

We offer you a professional and scientific support to conduct clinical trials and the decision making processes during the early phases of the development of new molecules. This includes clinical pharmacology trials giving an insight into a drug candidate’s safety, tolerability, pharmacokinetics and pharmacodynamics.

Looking for clinical advice or support, or preparing for a clinical study? We will help you set up a cost-effective and time-efficient study! Get in touch with our experts [info@b2h.be].


Induced pluripotent stem cells

Due to the close association with the CHU de Liège, GIGA researchers have access to fibroblasts of patients. They have developed a large expertise in the generation of induced pluripotent stem cells, their characterization and differentiation into specialized neuronal cells in vitro.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Stem Cells

Teams at GIGA are specialized in the investigations of stem cells (including mesenchymal stem cells and neural crest stem cells) biology, purification and expansion, as well as in their therapeutic use in the context of regenerative medicine.

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.

Novel pharmaceutical formulations

Researchers at ULg have a broad expertise in the development of novel pharmaceutical formulations to improve the solubility and the bioavailability of compounds and to facilitate drug delivery to brain cells.

To test these new formulations, they have also developed a cellular blood brain barrier model using immortalized human cerebral microvascular endothelial cells.

For more information, click here.

Interested in these projects? Drop us an email [info@b2h.be]. We are looking forward collaborating with you!

Patient diagnosis, treatment & follow-up

  • Electrical source imaging especially in cortical malformation-related epilepsy, a non-invasive pre-surgical investigation method for refractory focal epilepsy
  • Surgical treatment alternatives for refractory epilepsy such as:
    • Ablative strategy (resection of epileptogenic focus)
    • Inhibition of the spreading of epileptic discharges by neurostimulation (conservative/less invasive strategy)

Looking for clinical advice or support, or preparing for a clinical study? We will help you set up a cost-effective and time-efficient study! Get in touch with our experts [info@b2h.be].

EFHC1 in juvenile myoclonic epilepsy

Mutations in the EFHC1 gene have been found in several patients suffering from juvenile myoclonic epilepsy (JME). Researchers at GIGA have shown that EFHC1 loss of function disrupts cell division and induces apoptosis in cell culture.

Using ex vivo electroporation, they observed a dramatic decrease of radial migration because both division of cortical progenitors and locomotion of postmitotic neurons are impaired in the absence of the protein. Therefore, they propose that abnormal neuronal migration during development could lead to abnormal brain circuitry that, in turn, will produce JME.

Their goal is now to study the effect of EFHC1 and of its mutated forms on neocortical development and, subsequently, to study the functional consequences of the migratory deficits induced by EFHC1 loss of function.

Interested in these projects? Drop us an email [info@b2h.be]. We are looking forward collaborating with you!

Electrophysiology

electrophysiologyElectrophysiology project aims at a better understanding of the role of various types of ionic channels in the modulation of neuronal activity. More particularly, it helps to:

  • Evaluate the role of various ionic channels (SK K+ channels, M type K+ channels, voltage gated Na+, K+ and Ca²+ channels) in the control of the excitability of mesencephalic monoaminergic neurons
  • Better understand the biophysical properties of the proteins making up these channels
  • Discover new selective modulators of these channels, able to cross the blood-brain barrier, with the hope to design future interesting central nervous system medications

Researchers at GIGA have developed different types of recording such as:

  • Extracellular or intracellular recordings on rodent brain slices
  • Patch clamp on cell lines or rodent brain slices

Contact [info@b2h.be] us to discuss how these capabilities can forward your projects! We will help you develop tailored solutions.